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iSpot
 

AID iSpot Reader

 

 
   
 

 

Be prepared for the EliSpot future and analyse your enzymatic assays and your two-color fluorospot assays in the same innovative AID Elispot Reader.

Scanning and counting and analysing with only one software in just two minutes in the enzymatic mode. Fluorospot-count in just 6-7 minutes

  • Software driven filter cube
  • Only a mouse-click from enzymatic to fluorescent scanning and vice versa
  • Prepared for Cy3/FITC two-color analysis, other combinations on demand
  • Sophisticated new imaging technology to detect single- and double-stained spots with 100% accuracy

Ask for a lab demo now!

 

 
 
 
 

 

During the last years AID has established a collection of EliSpot Assay kits. These kits could be used for:

- Answering questions at the basic scientific level
- Detection of underlying infections with selected
pathogens.
- Monitoring of cytokine status during therapy

Therefore researchers as well as clinicians have access to an excellent tool for fast and reliable investigation of the parameters of host immunity.
AID has recently established a new iSpot reader  with dual capability (analysis of conventional colorimetric EliSpot assay as well as new fluorescence based dual color Fluorescent assays (Red/Cy3 and green /FITC labels).
Given that no hardware manipulation will be required for switching between colorimetric and fluorescent assay analysis, AID is now launching a new era of EliSpot technology!

A B
One mouse click enables the user to switch between fluorescent-dye and colorimetric
based analysis. No hardware manipulation is necessary.

A. Fluorescent-assay, showing three subsets of T-Cells.
Green: IFNy-secreting, Red IL2-secreting. Yellow: INFy/IL2-secreting.
B. Conventinal Assay, using the BCIP/NBT system.

Key Features:

  • Conventinal and fluorescent-based EliSpot analysis
  • Suitable for advanced TH1/TH2 balance analysis
  • Switch between both detection types within seconds
  • Improved multiple color analysis
  • Based on the proven AID EliSpot Reader System technology
  • Reads one 96well plate within 8-15 minutes
  • Software driven camera focus
  • Comes with the new software version 4.0
    • Digital Camera , FireWire, Color, CCD Chip (Digital cameras work without grabber cards; they are much faster and the images have better signal-to-noise characteristics. A digital camera will perform better than a video camera of nominally higher resolution. FireWire is a transfer system not unlike USB but much faster.)
    • Custom made optics
      (Custom- made for us by a German manufacturer of endoscopes and other high-grade optics, 6,5 mm display on 1/3" at 70 mm distance, optical resolution ~10 µm)
    • QuadPack 2-axis precision ballbearing stage, impact-protected
    • LED ring illumination, long-life
    • MS Windows XP Professional
    • Intel Core 2, 2 GB RAM min. 250 GB HD
    • Firewire host adaptor
    • DVD Writer, USB memory stick, networking card
    • High-quality colour photo printer
    • Screen: 19” TFT
    • AID Elispot Software 4.0
    • MS Office 2000/XP incl. Word and Excel
    • AID EliStat statistics plugin
    • more details...
    • Cable Set, Keyboard, Mouse
    • Handbook
    • Master plate on demand
  • Technical specifications:

    AID iSpot Reader Unit

    Computer/Control Unit

    Software

    Miscellaneous